buy Ubiquitin-conjugating enzyme E2C

Ubiquitin Conjugating enzyme E2C Human Recombinant

$115.00

  • Drug Discovery: Researchers use the recombinant protein to screen for small-molecule inhibitors that could potentially stop the uncontrolled cell division seen in tumors.

  • Cell Cycle Studies: It is a standard tool for scientists studying the transition from metaphase to anaphase during mitosis.

buy Ubiquitin-conjugating enzyme E2C online 

buy Ubiquitin-conjugating enzyme E2C (also known as UBE2C or UbcH10) is a critical protein in the human cell cycle. As a recombinant protein, it is widely used in laboratory research to study protein degradation, cell division, and cancer progression.

Core Biological Function

UBE2C is a member of the E2 family of ubiquitin-conjugating enzymes. Its primary role is to act as a “middleman” in the ubiquitination pathway:

  1. Activation: It accepts ubiquitin from an E1 activating enzyme.

  • Conjugation: It works with the Anaphase-Promoting Complex/Cyclosome (APC/C) (an E3 ligase).

  • Degradation: It attaches ubiquitin chains (specifically Lys-11 and Lys-48 linked) to target proteins like Cyclin A and Cyclin B. This signals the proteasome to destroy these proteins, allowing the cell to exit mitosis and complete division.

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Recombinant Protein Specifications

When purchased for research, the human recombinant version typically has the following characteristics:

Property Standard Details
Expression Host Commonly E. coli or HEK293 cells.
Molecular Weight Approximately 19.5 kDa to 21 kDa (may vary slightly depending on the tag).
Common Tags N-terminal His-tag (for purification) or GST-tag.
Purity Typically >90% or >95% as determined by SDS-PAGE.
Activity Validated for use in in vitro ubiquitination assays.
Storage Highly sensitive; usually stored at -80°C. Avoid freeze-thaw cycles.

Research & Clinical Significance

  • Cancer Biomarker: UBE2C is often overexpressed in various cancers (breast, lung, gastric, etc.). High levels are frequently associated with poor prognosis and higher tumor grades, making it a target for diagnostic research.

  • Drug Discovery: Researchers use the recombinant protein to screen for small-molecule inhibitors that could potentially stop the uncontrolled cell division seen in tumors.

  • Cell Cycle Studies: It is a standard tool for scientists studying the transition from metaphase to anaphase during mitosis.

Handling Precautions/buy Ubiquitin-conjugating enzyme E2C

If you are working with this protein in a lab:

  • Thaw on ice and mix gently; do not vortex, as this can denature the protein.

  • Aliquot the protein upon the first thaw to ensure you only freeze/thaw the amount needed for each experiment.

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